Isoquinolinesulfonamide Protein Kinase Inhibitors H7 and H8 Enhance the Effects of Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) on Neutrophil Function and Inhibit GM-CSF Receptor Internalization
نویسندگان
چکیده
Human granulocyte-macrophage colony-stimulating factor (GM-CSF) increases neutrophil surface expression of the cellular adhesion molecule CD11 b and primes the respiratory burst stimulated by the bacterial peptide f-met-leuphe (FMLP). W e have examined the effects of the isoquinolinesulfonamide protein kinase inhibitors H7 and H8 on these functions of GM-CSF using whole blood assays. Concentrations of H7 and H8 that inhibited the 12-0tetradecanoyl-phorbol-I 3-acetate (TPA) stimulated upregulation of CD11 b expression and activation of the respiratory burst, both augmented the effects of GM-CSF. H7 and H8 enhanced the GM-CSF-stimulated increase in CD11 b expression to 21 5% k 10% (P < .05) and 233% k 45% ( P < .05), respectively, of the value obtained with GM-CSF
منابع مشابه
Induction of Apoptosis on K562 Cell Line and Double Strand Breaks on Colon Cancer Cell Line Expressing High Affinity Receptor for Granulocyte Macrophage-Colony Stimulating factor (GM-CSF)
Background: Immunotoxins are comprised of both the cell targeting and the cell killing moieties. We previously established a new immunotoxin, i.e. Shiga toxin granulocyte macrophage-colony stimulating factor (StxA1-GM-CSF), comprises of catalytic domain of Stx, as a killing moiety and GM-CSF, as a cell targeting moiety. In this study, the ability of the immunotoxin to induce apoptosis and dou...
متن کاملImprovement in Development and Quality of 8 Cell Mouse Embryos in Presence of Granulocyte Macrophage-Colony Stimulating Factor
Purpose: Granulose Macrophage-Colony Stimulating Factor (GM-CSF) is a lympho- heamatopoietic actor, secreted in the reproductive system. Murine pre- implantation embryos express GM-CSF receptors. In his study, the capacity of eight cell mouse embryos was studied in the presence and absence of GM-CSF. Materials and Methods: Female NMRI mice were super ovulated using Pregnant Mare Serum gonadotr...
متن کاملA Functional Polymorphism of the Granulocyte Macrophage Colony Stimulating Factor is not Associated with the Outcome of HTLV-I Infection
Introduction Genetic background has known to be associated with the outcome of human T cell lymphotropic virus (HTLV) type I infection. In The present study we investigate the association between GM-CSF gene polymorphisms with the outcome of HTLV-I infection. Materials and Methods We analyzed 3 single-nucleotide polymorphisms in the promter region of granulocyte macrophage colony stimulating...
متن کاملInvolvement of tyrosine kinases in the activation of human peripheral blood neutrophils by granulocyte-macrophage colony-stimulating factor.
The aim of the present study is to evaluate the involvement of human neutrophil tyrosine kinase(s) in the signal transduction mechanism of granulocyte-macrophage colony-stimulating factor (GM-CSF). Stimulation of neutrophils with GM-CSF resulted in a time- and dose-dependent phosphorylation of several proteins having estimated molecular weights of approximately 40, 55, 74, 97, 118, and 155 Kd, ...
متن کاملHigh-yield Production of Granulocyte-macrophage Colony-stimulating Factor in E. coli BL21 (DE3) By an Auto-induction Strategy
A novel strategy to increase protein expression yield is unintended induction of expression in complex media, called auto-induction. This method can be used to express proteins under control of the lac promoter without any need to monitor bacterial growth pattern, and addition of specific expression inducers such as Isopropyl β-D-1-thiogalactopyranoside (IPTG) at proper time. In the present stu...
متن کامل